Purchase of the Maxwell TWO tor the Biotech Research and Innovation centre

Beskrivelse fra ordregiveren

The MaxWell Biosystems MaxTwo High-Density Microelectrode Array (HD-MEA) platform, including recording hardware, acquisition and analysis software, a dedicated workstation, and compatible multiwell HD-MEA consumables. The purpose of the purchase is to enable non-invasive electrophysiological recordings from neuronal and other excitable cell preparations in vitro with single-cell resolution. The system provides a high electrode density (3,265 electrodes/mm²) and low intrinsic noise levels (< 4 µVrms), supporting reliable detection of action potentials and downstream analyses such as spike sorting and single-unit discrimination. The small electrode dimensions (~12 µm) and small electrode pitch enable spatially resolved recordings and selective stimulation. A 24-well plate format is required to support parallel multi-condition experiments. The platform supports both 6-well and 24-well HD-MEA formats while maintaining comparable electrode density and recording performance. Each well provides a large sensing area (~8 mm²), enabling recordings from dense cultures, organoids, and tissue preparations. The system includes an integrated Axon Tracking Assay enabling analysis of axonal action potential propagation, conduction velocity, and label-free assessment of axonal morphology. The platform allows user-defined selection of stimulation electrodes within the multiwell configuration, supporting adaptable stimulation protocols. For experiments requiring continuous medium exchange, the system can be combined with an integrated perfusion system compatible with the 6-well format, enabling parallel recording of up to six brain slices. Overall, the MaxTwo HD-MEA platform is required to support current and planned research at the University of Copenhagen by enabling scalable multiwell single-cell electrophysiology and perfusion-compatible experimental workflows within one integrated system.

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